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Two viral gene targets needed in SARS-CoV-2 detection by PCR, say researchers – News-Medical.Net

Researchers at the Chan Zuckerberg Biohub, San Francisco, and collaborators have warned against only targeting one viral gene when carrying out reverse transcriptase-polymerase chain reaction (RT-PCR) testing for severe acute respiratory syndrome coronavirus …

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Researchers at the Chan Zuckerberg Biohub, San Francisco, and collaborators have warned against only targeting one viral gene when carrying out reverse transcriptase-polymerase chain reaction (RT-PCR) testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
The team conducted a study showing that a mutation had arisen in the N gene of the virus that impaired the annealing of a commonly used RT-PCR primer.
Manu Vanaerschot and colleagues say the World Health Organization (WHO) …

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